[Based on the gut microbiome, an examination of the mechanisms of the cyanocytopenic mouse].
An international medical study has brought new findings and related findings have important reference value for disease control. This study aims to investigate the therapeutic effects of Tianshu capsules (TS) on the Migrainerat model and explore its potential pharmacological mechanisms. A Migrainerat model was established by injecting glycosidic acid into the skin.
The Sprague-Duvalier rat was randomly assigned to control groups, experimental groups, low-dose TS groups, dose TS groups and high-dose TS groups. The expression of the coding factor cfos gene in the trigeminal nerve nodes was measured using quantitative real-time polymerase chain reaction (PCR) methods. In human serum, the levels of the coding factor cfos gene (CGRP) and the thyroid hormone-related gene (5-HT) were both measured by the enzyme-linked immunosuppression test (ELISA).
Experimental results showed that TS significantly improved the abnormality of blood sugar in mice, improved facial pain thresholds, and improved biological behaviour indicators such as thresholds. It also significantly suppressed the abnormal expression of the gene c-fos in the trigeminal nerve and spinal fluid. Metabolic group beta-diversity analysis and differential species abundance analysis revealed significant gut microbiome mismatch induced by migraine models, characterized by an increase in the abundance of harmful microbiome groups, including a decrease in the abundance of beneficial microbiome groups such as Streptococcus and Entercoccus.
The results of the KEGG functional pathway analysis further revealed a significant decrease in the relative abundance of the major pathways associated with the biosynthesis of uranoates, tyranoates, and seranoates in the model group. TS intervention increased the abundance of the genus (such as Parabacteroides, Eubacterium, Allobaculum, and Muribaculum), such as the increase in the number of species in the genus Parabacteroides. At the same time, the TS intervention also significantly improved the amino acid metabolic pathways associated with barrier function (such as the tight junction) and pathways associated with biosynthesis pathways.
In addition, the TS intervention also significantly improved the amino acid metabolic pathways and pathways associated with biosynthesis pathways. It should be noted that the above information is based on international public research findings compiled for reference by HealthCop and does not constitute clinical advice.
